Human Papilloma Virus and Epstein-Barr Virus in Sinonasal inverted Papilloma

Background: Schneiderian papillomas are uncommon benign tumors of the sinonasal area. They tend to present local aggressiveness and recurrence, and some undergo malignant progression. This study aimed to search of humanpapillomavirus (HPV) and Epstein–Barr virus (EBV) in sinonasal inverted papilloma (IP) in order to elucidate possible role in its pathogenesis. Methods: Forty-eight IPs were subjected to chromogenic in-situ hybridization (CISH) for HPV DNA (HPV-III family16), Real-time PCR for HPV, p16, anti-EBV and Ki67 immunohistochemical (IHC) studies. Results: p16 was positive in 30 of 48 (62.5%), CISH for HPV was positive in 1 of 48 IPs (2.1%). All specimens were EBV negative. In total, 33.3% of IPs showed suprabasal Ki67 reactivity. HPV prevalence of our IP is high. EBV is not present in IP. Conclusion: This evidence suggested that HPV infection but not EBV plays a role in pathogenesis of IP. Negative PCR results possibly depend on age of the paraffin blocks.